Effects of taxol on in-vitro matured bovine oocyte vitrification

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The effects of pre and post vitrification taxol treatment on bovine immature oocyte

The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (COCs). Bovine ovaries were collected from the local slaughterhousein a container with 30-37°C physiologic saline and supplemented with antibiotics. The grade A aspiratedCOCs were assigned to 6 experimental groups; The 1st group was control (n=100) in whi...

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the effects of pre and post vitrification taxol treatment on bovine immature oocyte

the aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (cocs). bovine ovaries were collected from the local slaughterhousein a container with 30-37°c physiologic saline and supplemented with antibiotics. the grade a aspiratedcocs were assigned to 6 experimental groups; the 1st group was control (n=100) in whi...

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Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes

This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of choles...

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Effect of Demecolicne Treatment on The Developmental Competency of In Vitro Matured Bovine Oocytes

Purpose: The aim of this study was to investigate whether demecolicne treatment of matured bovine oocytes adversely affects the process of in vitro fertilization and embryo development. Materials and Methods: Bovine Cumulus Oocyte Complexes (COC's) were matured in vitro and then were randomly allocated to two treatment groups of common concentrations of demecolicne (0.05 and 0.4 mg/ml for 30 mi...

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P-118: Effect of Vitrification on Developmental Competence of Parthenogenetic Activation in In Vitro Matured Ovine Oocytes

Background: Cryopreservation of in vitro matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies. On the other hand, the cryopreservation of oocytes is an open problem as a result of their structural sensitivity to the freezing process. The purpose of this study was to evaluate the effect of vitrification on in vitro development of vitri...

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ژورنال

عنوان ژورنال: TAP CHI SINH HOC

سال: 2012

ISSN: 0866-7160,0866-7160

DOI: 10.15625/0866-7160/v34n3se.1802